Computational and Mathematical Methods in Medicine
Volume 2013 (2013), Article ID 162105, 6 pages
Research Article

Three-Dimensional Identification of Microorganisms Using a Digital Holographic Microscope

1Shenzhen Key Lab of Wind Power and Smart Grid, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen 518055, China
2School of Mechanical and Electrical Engineering, Harbin Institute of Technology, 92 West Dazhi Street, Nan Gang District, Harbin 150001, China
3GRG Banking Equipment Co., Ltd., 9 Kelin Road, Science Town, Guangzhou 510663, China

Received 4 February 2013; Accepted 6 March 2013

Academic Editor: Shengyong Chen

Copyright © 2013 Ning Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This paper reports a method for three-dimensional (3D) analysis of shift-invariant pattern recognition and applies to holographic images digitally reconstructed from holographic microscopes. It is shown that the sequential application of a 2D filter to the plane-by-plane reconstruction of an optical field is exactly equivalent to the application of a more general filter with a 3D impulse response. We show that any 3D filters with arbitrary impulse response can be implemented in this way. This type of processing is applied to the two-class problem of distinguishing different types of bacteria. It is shown that the proposed technique can be easily implemented using a modified microscope to develop a powerful and cost-effective system with great potential for biological screening.